Extraction of DNA from E.coli bacteria

I chose to research the extraction of DNA from E. coli bacteria because by extracting DNA it will allow us to identify its genetic characteristics to develop proven and reliable methods of diagnosis and of course to find new medications for it.  The more knowledge we have on this microbe the faster we can correctly identify it and start to treat it.

My plan for researching this project will be to use five different protocols for extracting DNA from the bacteria E. coli .  The first protocol that will be conducted is a high quality commercial extraction kit that gives high DNA yields. The other four protocols are the boiling, meat tenderizer, freeze-thaw and egg whites protocols.  These remaining four protocols will be conducted and compared to the first protocol of high quality commercial kit to see which one will produce comparable DNA extraction yields.  

My hypothesis is that out of the four remaining protocols tested the freeze thaw protocol will produce a comparable DNA yield to that of the high quality commercial DNA extraction kit.  We'll see what happens I'm excited to find out.

Here is a diagram of the freeze and thaw protocol that I will be doing next week: