Creating Poster for Presentation

This week’s lab hours were dedicated to completing my presentation poster. This is my first research poster and it will also be my first time presenting.  I’m looking forward to coming full circle in this S-Stem program, complete my research project and discuss with others what I’ve learned in the process.  Below are portions of the poster completed. 

Summary of Results

Data collected was inconsistent, the following observations were made during the experiment process that could account for the inconsistencies in the data.  In centrifuge tube 4/7/17 Egg White 2 a gnat was found trapped inside contaminating the sample.  Centrifuge tubes were not all flicked/tapped to mix contents evenly before adding samples on the Nano Drop Machine and obtaining data.  The final volume in each centrifuge tube was not consistent due to different size DNA cell pellets formed when centrifuged. This is possibly due to insufficient incubation time of Providencia stuartii TSB, used in the extraction of DNA.  The final observation made was of the DNA yield numbers in the commercial kit samples, they were lower than expected.  This was due to the low concentration of the Ready-Lyse-Lysozyme being used, to correct this the concentration was increased.    

Conclusion

Providencia stuartii DNA yields extracted using egg white lysozyme was detected in samples collected the day of and samples that were 1, 6, and 8 days old.  

The method used in this experiment of using a Nano Drop machine yielded data that was too inconsistent to make a definitive conclusion. 

The results of this study indicated the importance of technique application and that of using more than one method to analyze data in order to have consistent and accountable data.

Further testing using different methods such as PCR and/or gel electrophoresis should be done to further analyze the data and make a definitive conclusion.